UNPA Digital
Repositorio institucional de la Universidad del Papaloapan
Comunidades UNPA
Seleccione una comunidad para explorar sus colecciones.
Envíos recientes
ARTÍCULO Functional Yogurt Enriched with Moringa oleifera Leaf Extract: Physicochemical, Sensory, Microbiological, and Antihyperglycemic Evaluation in Diabetic Rats(Molecules, 2026-08-09) Rodríguez Romero, Beatriz Adriana; Paniagua Vega, David; Flores Maldonado, Orlando Esaú; Garza Juárez, Aurora de Jesús; Huerta Heredia, Ariana Arlene; Silva-Flores, P. G.Functional foods enriched with plant-derived bioactive compounds have emerged as promising complementary dietary strategies to improve glycemic regulation. The aim of this study was to evaluate the physicochemical properties, sensory acceptance, and microbiological characteristics of a functional yogurt enriched with Moringa oleifera leaf extract, as well as its effect on blood glucose levels in an alloxan-induced diabetic rat model. Phytochemical characterization of the hydroethanolic leaf extract by HPLC and direct-infusion ESI-IT-MS2 revealed phenolic compounds, including chlorogenic acid, rutin, quercetin, and kaempferol, together with additional glycosylated flavonoids. M. oleifera extract increased the yogurt’s protein content. Sensory evaluation indicated moderate acceptance compared to natural yogurt, with color being the least-accepted attribute. Microbiological analysis showed no inhibitory effect against representative bacterial genera of the gastrointestinal microbiota under the experimental conditions evaluated. In vivo evaluation in alloxan-induced diabetic female Wistar rats demonstrated a significant reduction in blood glucose levels, from 396.50 ± 17.85 mg/dL to 254.50 ± 32.79 mg/dL after 15 days of treatment. These findings support further investigation of M. oleifera functional yogurt as a potential functional food in the context of glycemic control.ARTÍCULO NMR and Chemometric Analysis of Verbascoside and Isoverbascoside Produced in Tecoma stans In Vitro Cultures(Magnetic Resonance in Mexico and Central America, 2025-05-29) Paniagua Vega, David; Huerta Heredia, Ariana Arlene; Sánchez Otero, María Guadalupe; Waksman, Noemí; Lucio-Gutiérrez, Ricardo; Saucedo, Alma L.Verbascoside and isoverbascoside are phenylethanoid glycosides with reported biological activities such as neuroprotection, hepatoprotection, anti-inflammatory, antimicrobial, anticancer, and antioxidant properties. These compounds are constitutively present in roots, stems, leaves, and flowers of various plant species, including Tecoma stans. In Mexico, this plant is traditionally used as a safe and effective herbal treatment for chronic diseases and its complications including diabetes and renal and hepatic disorders. The potential pharmacological applications of verbascoside and isoverbascoside have conducted efforts to produce these compounds in cell and plant tissue cultures. In this study, T. stans root and plantlet in vitro cultures were established as potential sources of verbascoside and isoverbascoside, and NMR was used as primary analytical tool. As a first step, proton and bidimensional NMR analysis confirmed the presence of verbascoside and isoverbascoside in T. stans in vitro culture extracts. Subsequently, their contents were quantified by means of quantitative NMR (qNMR) based on the external standard PULCON method. Furthermore, 1H-NMR spectral data were used to develop a descriptive PLS-DA model, which confirms the qNMR results. This model indicated that differences in the amounts and proportions of verbascoside and isoverbascoside produced by roots and plantlets are the primary factors in distinguishing these samples. These results demonstrate the capability of T. stans in vitro systems as biotechnological tools for obtaining phenylethanoids with high pharmacological potential and confirm the broad applicability of NMR as an analytical platform. However, additional experiments are necessary to improve the phenylethanoids glucoside yields and support the validation of the qNMR method.Profesor Huerta Heredia, Ariana ArleneProfesor InvestigadorARTÍCULO Verbascoside and isoverbascoside production from the in vitro root culture of Tecoma stans(Plant Biosystems, 2026-02-03) Paniagua Vega, David; Anaya Esteban, Eduardo; Cisneros Ríos, D. J.; Navarro Martinez, Ana Karin; Barrera Figueroa, Blanca Estela; Rivas-Galindo, Verónica M.; Huerta Heredia, Ariana ArleneTecoma stans is a notable source of antioxidant, antibacterial, antiviral, and anticancer agents, and several of its biological actions are associated with the accumulation of secondary metabolites, such as alkaloids and phenolics. In this study, we developed an in vitro root culture of T. stans under photoperiod condition and showed its growth kinetics, demonstrating the verbascoside (Ve) and isoverbascoside (IVe) accumulation. Electrospray ionization and ion-trap analyzer (DFI-ESI-IT-MSn) found both molecules, including caffeic acid, caffeic acid hexoside, and caffeic acid pentoside. In vitro root culture was produced, and a peak biomass occurred at 40 d; however, maximum Ve accumulation was observed at 20 d (4.65 ± 0.35 mg/gDW), and IVe accumulation peaked at day 40. The maximum flavonoid and phenolic compound accumulation was quantified on the 20th day. A linear association between biomass increment (∆B) and the change in electrical conductivity (∆EC) was established as ΔEC = 0.31∆B + 0.15, R2 = 0.96, functioning as a fast and indirect growth measurement. To the best of our knowledge, this is the first report on T. stans root culture that demonstrates its biosynthetic ability to produce Ve and IVe.ARTÍCULO Growth of transformed Stevia rebaudiana in in vitro systems and the stimulation of steviol glycoside production with soy protein hydrolysates(Plant Cell, Tissue and Organ Culture, 2026-07-25) Alvarado Orea, Itzel Vianney; Atilano González, Noelia; Villalobos Amador, Enrique; Peña Castro, Julián Mario; Robledo-Narvaez, Paula N.; Ortega-Clemente, L. A.; Paniagua Vega, David; Huerta Heredia, Ariana ArleneIn this study, morphological characteristics and secondary metabolite production were explored in Agrobacterium rhizogenes-transformed plantlets of Stevia rebaudiana growing in semi-solid (SS) and liquid media (LM) in flasks or a twin-bottle temporary immersion system (TIS). The effect of adding 0.75 and 1.5 mL/L of soy protein hydrolysates (SPH) in LM was also evaluated. In plantlet growing without SPH, more shoots and leaves were obtained in SS media. Shoots grown in TIS were longer than those grown in flasks and SS media. Biomass accumulation was greater in TIS than in the other growth systems. Total phenolic content (TPC) and steviol glycosides production were higher in plants grown in the SS media than in those grown in LM. SPH improved the morphological characteristics of S. rebaudiana plantlets. Plants growing in flasks with 0.75 mL/L SPH had increased shoots, total chlorophyll, and leaf area compared with the control. Plants grown in TIS with 1.5 mL/L SPH exhibited improved foliar biomass, leaf area, and chlorophyll content. The addition of SPH to liquid culture media, in both flasks and TIS, enhanced stevioside (Ste) and rebaudioside A (Reb A) content in seedlings in comparison to the controls. Incorporating 0.75 mL/L SPH increased the Reb A: Ste ratio to 1.44. TPC only increased in flasks with 1.5 mL/L SPH. The highest Ste, Reb A, and TPC yields were obtained from seedlings grown in the TIS. SPH and TIS improved the yield and quality of S. rebaudiana plantlets.