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Biochemical and Kinetic Characterization of the a-Amylase from Bacillus amyloliquefaciens JJC33M, AmyJ33, On Raw Starch

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dc.contributor.authorHernández-Heredia, Sarahi
dc.contributor.authordel Moral Ventura, Sandra Trinidad
dc.contributor.otherInstituto de Biotecnología
dc.date.accessioned2026-02-03T17:43:39Z
dc.date.issued2016-11-01
dc.description.abstractAmyJ33 is an a-amylase from Bacillus amyloliquefaciens JJC33M is not calcium dependent and is active on native and raw starch. In this work, a deeper biochemical and kinetic characterization was made. AmyJ33 presented an optimum pH and temperature at 5 and 80°C, respectively, it was stable at pH 5 and a had half-life of 16h (40°C), 2.25 h (50°C) and 0.25 h (60°C). The enzyme produces glucose, maltose and maltopentose traces of potato starch, in 180 min it hydrolyzed 25% of raw starch via exo-corrosion. Km and Vmax values were 10.6 mg/mL and 41.0 U/mg, respectively. AmyJ33 could be used in the baking industry or to produce ethanol on raw starch.
dc.identifier.citationHernández-Heredia, S., & del Moral, S. (2016). Biochemical and kinetic characterization of the a-amylase from Bacillus amyloliquefaciens JJC33M, AmyJ33, on raw starch. JSM Biotechnology & Biomedical Engineering, 3(5), 1–7.
dc.identifier.issn2333-7117
dc.identifier.urihttps://repositorio.unpa.edu.mx/handle/10598/650
dc.identifier.urlhttps://www.jscimedcentral.com/jounal-article-info/JSM-Biotechnology-and-Biomedical-Engineering/Biochemical-and-Kinetic-Characterization-of-the-a-Amylase-from-Bacillus-amyloliquefaciens-JJC33M,-AmyJ33,-On-Raw-Starch-2170#
dc.languageInglés
dc.publisherJSM Biotechnology and Biomedical Engineering
dc.relation.ispartofJSM Biotechnology and Biomedical Engineering, Vol. 3, Núm. 5
dc.rightsAcceso abierto
dc.rights.holderJSciMed
dc.subjectα-amilasa
dc.subjectAlmidón crudo
dc.subjectBacillus amyloliquefaciens JJC33M
dc.titleBiochemical and Kinetic Characterization of the a-Amylase from Bacillus amyloliquefaciens JJC33M, AmyJ33, On Raw Starch
dc.typeArtículo
dspace.entity.typePublication
relation.isOrgUnitOfPublicationb033d851-c044-43df-abe6-359a6bc51484
relation.isOrgUnitOfPublication.latestForDiscoveryb033d851-c044-43df-abe6-359a6bc51484

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